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MUK 4.2.992-00

МУК 4.2.992-00

E. coli O157: H7 Methods for Isolation and Identification of Enterohemorrhagic E. coli

Методы выделения и идентификации энтерогеморрагической кишечной палочки E. coli O157 : H7

Status: Effective - Introduced for the first time

Methodological guidelines determine the methods for detecting, isolating and identifying enterohemorrhagic E. coli E. coli O157: H7, are mandatory for controlling baby food, dairy and meat products during anti-epidemic measures during outbreaks, as well as sanitary and epidemiological surveillance bacteriological laboratories of state sanitary and epidemiological surveillance centers, other institutions of the RF health care system that carry out bacteriological diagnostics stryh intestinal infections of bloody and development of hemolytic uremic syndrome, as well as a survey of contact in the foci of the disease and the control over the quality of food raw materials and food products.

Методические указания определяют методы обнаружения, выделения и идентификации энтерогеморрагической кишечной палочки E. coli O157 : H7, являются обязательными при контроле продуктов детского питания, молочных и мясных продуктов в ходе проведения противоэпидемических мероприятий при возникновении вспышек, а также осуществления санитарно-эпидемиологического надзора и предназначены для бактериологических лабораторий центров госсанэпиднадзора, других учреждений системы здравоохранения РФ, осуществляющих бактериологическую диагностику острых кишечных инфекций с гемоколитом и развитием гемолитико-уремического синдрома, а также обследование контактных в очагах заболевания и контроль за качеством продовольственного сырья и пищевых продуктов.

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Format: Electronic (pdf/doc)

Approved: Chief State Sanitary Doctor of the Russian Federation, First Deputy Minister of Health of the Russian Federation, 11/4/2000

SKU: RUSS160009

Price: $343.00



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The Product is Contained in the Following Classifiers:

Evidence base (TR CU, Technical Regulation of the Customs Union) » 021/2011 TR CU. About food safety » Regulations and standards (to ТР ТС ТС 021/2011) »

PromExpert » SECTION I. TECHNICAL REGULATION » V Testing and control » 4 Testing and control of products » 4.4 Testing and control of food products »

ISO classifier » 67 PRODUCTION OF FOOD » 67.050 General methods for food inspection and analysis »


The Document References:

GOST 10444.1-84: Canned food. Preparation of reagent solutions, dyes, indicators and culture media for microbiological analysis

GOST 12026-76: Filter paper for laboratory application

GOST 13646-68: Glass mercury thermometers designed for precise measurements

GOST 13739-78: Immersion oil for microscopy. Technical requirements. Methods of testing

GOST 13805-76: Dry fermentative peptone designed for bacteriologic purposes

GOST 14919-83: Household electric ranges, electric plates and electric frying ovens

GOST 17206-84: Agar for microbiological investigations

GOST 19569-89: Steam medical sterilizers

GOST 21237-75: Meat. Methods of bacteriological analysis

GOST 21240-89: Medical scalpels and knives

GOST 21241-89: Medical pincers

GOST 23932-90: Laboratory glassware and equipment. General specifications

GOST 24104-88: General purpose and reference laboratory balances

GOST 2493-75: Reagents. Potassium phosphate, dibasic 3-aqueous. Specifications

GOST 25336-82: Laboratory glassware and equipment. Basic parameters and dimensions

GOST 26668-85: Food-stuff and food additives. Preparation of sampling for microbiological analyses

GOST 26669-85: Food-stuffs and food additives. Preparation of samples for microbiological analyses

GOST 26670-91: Food products. Methods for cultivation of microorganisms

GOST 29227-91: Laboratory glassware. Graduated pipettes. Part 1. General requirements

GOST 30518-97: Food products. Methods for detection and quantity determination of coliformes

GOST 30519-97: Food products. Method for detection of Salmonella

GOST 3145-84: Mechanical clocks with signal. General specifications

GOST 4172-76: Reagents. Disodium hydrogen phosphate dodecahydrate. Specification

GOST 4198-75: Reagents. Potassium dihydrogen phosphate. Specifications

GOST 4208-72: Reagents. Ammonium ferrous sulphate. Specifications

GOST 4233-77: Reagents. Sodium chloride. Specifications

GOST 4288-76: Culinary products and half-finished products of minced meat. Rules of acceptance and test methods

GOST 5556-81: Medical absorbent cotton

GOST 5833-75: Reagents. Saccharose. Specifications

GOST 5962-67: Rectified ethyl alcohol. Specifications

GOST 6038-79: Reagents. D-glucose. Specifications

GOST 6672-75: Cover glasses for micropreparations. Specifications

GOST 6709-72: Distilled water. Specifications

GOST 6824-76: Distilled glycerine. Specifications

GOST 7702.2.2-93: Poultry meat, edible offal, ready-to-cook products. Methods for detection and quantity determination of coliform bacteria (Escherichia, Citrobacter, Enterobacter, Klebsiella, Serratia)

GOST 7702.2.3-93: Poultry meat, edible offal, ready-to-cook products. Method for detection of Salmonellae

GOST 9147-80: Laboratory porcelain ware and apparatus. Specifications

GOST 9225-84: Milk and milk products. Methods of microbiological analysis

GOST 9245-79: Measuring direct current potentiometers

GOST 9792-73: Sausage products and products of pork, mutton, beef and meat of other kinds of slaughter animals and poultry. Acceptance rules and sampling methods

GOST 9958-81: Sausage products and meat products. Methods of bacteriological analysis

The Document is Referenced By:

MR 02.036-08: Identification of pathogenic bacteria isolated during food control using the BAX System Q7

MR 4.2.0079/1-13: Organization of laboratory diagnosis of infectious diseases, laboratory monitoring of environmental objects during mass events

MUK 4.2.2872-11: Methods for identifying and identifying pathogenic bacteria that cause infectious diseases with food by transmission in PCR-based food products with hybridization-fluorescence detection. Methodical instructions

MUK 4.2.2963-11: Guidelines for the laboratory diagnosis of diseases caused by Escherichia coli, producing shiga-toxins (STEC-culture), and the detection of pathogens of STEC-infections in food

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